Review



upright confocal microscope nikon instruments eclipse ni-e with a c2 confocal laser scanner  (Nikon)


Bioz Verified Symbol Nikon is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Nikon upright confocal microscope nikon instruments eclipse ni-e with a c2 confocal laser scanner
    Validation of cell–matrix interaction with different cell lines stably expressing OptoIntegrin. a HEK-293T, HeLa and MCF7 cells stably expressing OptoIntegrin or moxGFP-PIF S were seeded on PhyB 1–651 -coated glass slides and incubated under 660 nm or 740 nm light (I = 20 µmol m −2 s −1 ) for 5 h and subsequently imaged using a transmission light <t>microscope</t> (scale bar = 200 µm). b Live cell imaging of cell–matrix interaction under 660 nm light and then switch to 740 nm light after 35 min with binary images of cell shapes to illustrate the cells spreading. For this, HeLa cells stably expressing OptoIntegrin were seeded on OptoMatrix and subsequently imaged. Micrographs were taken at indicated time points (scale bar = 10 µm). c Spatial control of cell attachment with OptoIntegrin-expressing HEK-293T cells. OptoIntegrin-expressing cells were cultivated on OptoMatrix, locally illuminated with 660 nm or 740 nm for 3 min and then left in darkness for 4 h. Afterwards, cells were fixed and imaged (scale bar = 200 µm)
    Upright Confocal Microscope Nikon Instruments Eclipse Ni E With A C2 Confocal Laser Scanner, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/confocal+microscope+nikon+eclipse+ni-e+c2/pmc06325061-167-24-27
    Average 90 stars, based on 1 article reviews
    upright confocal microscope nikon instruments eclipse ni-e with a c2 confocal laser scanner - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Optogenetic control of integrin-matrix interaction"

    Article Title: Optogenetic control of integrin-matrix interaction

    Journal: Communications Biology

    doi: 10.1038/s42003-018-0264-7

    Validation of cell–matrix interaction with different cell lines stably expressing OptoIntegrin. a HEK-293T, HeLa and MCF7 cells stably expressing OptoIntegrin or moxGFP-PIF S were seeded on PhyB 1–651 -coated glass slides and incubated under 660 nm or 740 nm light (I = 20 µmol m −2 s −1 ) for 5 h and subsequently imaged using a transmission light microscope (scale bar = 200 µm). b Live cell imaging of cell–matrix interaction under 660 nm light and then switch to 740 nm light after 35 min with binary images of cell shapes to illustrate the cells spreading. For this, HeLa cells stably expressing OptoIntegrin were seeded on OptoMatrix and subsequently imaged. Micrographs were taken at indicated time points (scale bar = 10 µm). c Spatial control of cell attachment with OptoIntegrin-expressing HEK-293T cells. OptoIntegrin-expressing cells were cultivated on OptoMatrix, locally illuminated with 660 nm or 740 nm for 3 min and then left in darkness for 4 h. Afterwards, cells were fixed and imaged (scale bar = 200 µm)
    Figure Legend Snippet: Validation of cell–matrix interaction with different cell lines stably expressing OptoIntegrin. a HEK-293T, HeLa and MCF7 cells stably expressing OptoIntegrin or moxGFP-PIF S were seeded on PhyB 1–651 -coated glass slides and incubated under 660 nm or 740 nm light (I = 20 µmol m −2 s −1 ) for 5 h and subsequently imaged using a transmission light microscope (scale bar = 200 µm). b Live cell imaging of cell–matrix interaction under 660 nm light and then switch to 740 nm light after 35 min with binary images of cell shapes to illustrate the cells spreading. For this, HeLa cells stably expressing OptoIntegrin were seeded on OptoMatrix and subsequently imaged. Micrographs were taken at indicated time points (scale bar = 10 µm). c Spatial control of cell attachment with OptoIntegrin-expressing HEK-293T cells. OptoIntegrin-expressing cells were cultivated on OptoMatrix, locally illuminated with 660 nm or 740 nm for 3 min and then left in darkness for 4 h. Afterwards, cells were fixed and imaged (scale bar = 200 µm)

    Techniques Used: Biomarker Discovery, Stable Transfection, Expressing, Incubation, Transmission Assay, Light Microscopy, Live Cell Imaging, Control, Cell Attachment Assay

    Related Articles

    Microscopy:

    Article Title: Similarity of activated sludge and treated wastewater with special reference to nitrifiers and their seasonal variability
    Article Snippet: .. The study was carried out on a Nikon Eclipse Ni-E C2 confocal microscope equipped with UV-2A, B-2A, and G-2A filters (with excitation (EX) wavelengths of 330–380, 450–490, and 510–560 nm, respectively) and Nis-Elements AR 4.30 software. ..

    Article Title: A novel ENTH domain-containing protein TgTEPSIN is essential for structural maintenance of the plant-like vacuolar compartment and bradyzoite differentiation in toxoplasma gondii.
    Article Snippet: Toxoplasma gondii is an intracellular and parasitic protozoon that harbors specialized cellular structures and molecular mechanisms, including the Plant-like Vacuolar Compartment (PLVAC).. The PLVAC performs multifaceted roles in the parasite, contributing to ion homeostasis, proteolysis, pH regulation, and autophagy.. Despite significant efforts over the past decade to characterize the PLVAC, the proteins localized to this organelle remain largely unidentified.

    Article Title: Generation of Functional and Mature Sympathetic Neurons from Human Pluripotent Stem Cells via a Neuroepithelial Route.
    Article Snippet: The sympathetic nervous system (SNS) is a crucial branch of the autonomic nervous system (ANS) that is responsible for regulating visceral function and various physiological processes.. Dysfunction of the SNS can lead to various diseases, such as hypertension and metabolic disorders.. However, obtaining sympathetic neurons from human tissues for research is challenging.

    Article Title: Human skeletal muscle aging atlas
    Article Snippet: .. Images were taken with Nikon C2 Confocal Microscope (Nikon Eclipse Ni-E) and DMi8 inverted microscope (Leica Microsystems). .. Next, muscles were blocked with 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) diluted in 0.5% PBST containing 3% BSA for 60 min. Immunofluorescence was performed with primary antibodies (Supplementary Table 9) mixed with 2.5 μg/mL (performed under the Biosafety Cabinet) Cy3 conjugated αBungarotoxin (α-BTX, BosunLife, #00018) overnight at 4°C.

    Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike.
    Article Snippet: Afterward, the cell-climbing slice was mounted on microscope slides with antifade mounting medium (H-1000-10, Vector). .. Fluorescence images were captured using a Nikon C2 confocal microscope (Nikon Eclipse Ni-E), and colocalization analysis was performed using ImageJ software (ImageJ_v1.8.0) with the Plot Profile function. ..

    Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike
    Article Snippet: Afterward, the cell-climbing slice was mounted on microscope slides with antifade mounting medium (H-1000-10, Vector). .. Fluorescence images were captured using a Nikon C2 confocal microscope (Nikon Eclipse Ni-E), and colocalization analysis was performed using ImageJ software (ImageJ_v1.8.0) with the Plot Profile function. ..

    Article Title: Similarity of activated sludge and treated wastewater with special reference to nitrifiers and their seasonal variability.
    Article Snippet: .. The study was carried out on a Nikon Eclipse Ni-E C2 confocal microscope equipped with UV-2A, B-2A, and G2A filters (with excitation (EX) wavelengths of 330-380, 450-490, and 510-560 nm, respectively) and Nis-Elements AR 4.30 software. ..

    Software:

    Article Title: Similarity of activated sludge and treated wastewater with special reference to nitrifiers and their seasonal variability
    Article Snippet: .. The study was carried out on a Nikon Eclipse Ni-E C2 confocal microscope equipped with UV-2A, B-2A, and G-2A filters (with excitation (EX) wavelengths of 330–380, 450–490, and 510–560 nm, respectively) and Nis-Elements AR 4.30 software. ..

    Article Title: A novel ENTH domain-containing protein TgTEPSIN is essential for structural maintenance of the plant-like vacuolar compartment and bradyzoite differentiation in toxoplasma gondii.
    Article Snippet: Toxoplasma gondii is an intracellular and parasitic protozoon that harbors specialized cellular structures and molecular mechanisms, including the Plant-like Vacuolar Compartment (PLVAC).. The PLVAC performs multifaceted roles in the parasite, contributing to ion homeostasis, proteolysis, pH regulation, and autophagy.. Despite significant efforts over the past decade to characterize the PLVAC, the proteins localized to this organelle remain largely unidentified.

    Article Title: Similarity of activated sludge and treated wastewater with special reference to nitrifiers and their seasonal variability.
    Article Snippet: .. The study was carried out on a Nikon Eclipse Ni-E C2 confocal microscope equipped with UV-2A, B-2A, and G2A filters (with excitation (EX) wavelengths of 330-380, 450-490, and 510-560 nm, respectively) and Nis-Elements AR 4.30 software. ..

    Staining:

    Article Title: A novel ENTH domain-containing protein TgTEPSIN is essential for structural maintenance of the plant-like vacuolar compartment and bradyzoite differentiation in toxoplasma gondii.
    Article Snippet: Toxoplasma gondii is an intracellular and parasitic protozoon that harbors specialized cellular structures and molecular mechanisms, including the Plant-like Vacuolar Compartment (PLVAC).. The PLVAC performs multifaceted roles in the parasite, contributing to ion homeostasis, proteolysis, pH regulation, and autophagy.. Despite significant efforts over the past decade to characterize the PLVAC, the proteins localized to this organelle remain largely unidentified.

    Inverted Microscopy:

    Article Title: Human skeletal muscle aging atlas
    Article Snippet: .. Images were taken with Nikon C2 Confocal Microscope (Nikon Eclipse Ni-E) and DMi8 inverted microscope (Leica Microsystems). .. Next, muscles were blocked with 5% normal goat serum (Jackson ImmunoResearch, #005-000-121) diluted in 0.5% PBST containing 3% BSA for 60 min. Immunofluorescence was performed with primary antibodies (Supplementary Table 9) mixed with 2.5 μg/mL (performed under the Biosafety Cabinet) Cy3 conjugated αBungarotoxin (α-BTX, BosunLife, #00018) overnight at 4°C.

    Fluorescence:

    Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike.
    Article Snippet: Afterward, the cell-climbing slice was mounted on microscope slides with antifade mounting medium (H-1000-10, Vector). .. Fluorescence images were captured using a Nikon C2 confocal microscope (Nikon Eclipse Ni-E), and colocalization analysis was performed using ImageJ software (ImageJ_v1.8.0) with the Plot Profile function. ..

    Article Title: Nasal mucus-derived KLK13 restricts SARS-CoV-2 infection via proteolytic cleavage of spike
    Article Snippet: Afterward, the cell-climbing slice was mounted on microscope slides with antifade mounting medium (H-1000-10, Vector). .. Fluorescence images were captured using a Nikon C2 confocal microscope (Nikon Eclipse Ni-E), and colocalization analysis was performed using ImageJ software (ImageJ_v1.8.0) with the Plot Profile function. ..

    Periodic Counter-current Chromatography:

    Article Title: A novel ENTH domain-containing protein TgTEPSIN is essential for structural maintenance of the plant-like vacuolar compartment and bradyzoite differentiation in toxoplasma gondii.
    Article Snippet: Toxoplasma gondii is an intracellular and parasitic protozoon that harbors specialized cellular structures and molecular mechanisms, including the Plant-like Vacuolar Compartment (PLVAC).. The PLVAC performs multifaceted roles in the parasite, contributing to ion homeostasis, proteolysis, pH regulation, and autophagy.. Despite significant efforts over the past decade to characterize the PLVAC, the proteins localized to this organelle remain largely unidentified.



    Similar Products

    upright confocal microscope nikon instruments eclipse ni-e with a c2 confocal laser scanner  (Nikon)
    90
    Nikon upright confocal microscope nikon instruments eclipse ni-e with a c2 confocal laser scanner
    Validation of cell–matrix interaction with different cell lines stably expressing OptoIntegrin. a HEK-293T, HeLa and MCF7 cells stably expressing OptoIntegrin or moxGFP-PIF S were seeded on PhyB 1–651 -coated glass slides and incubated under 660 nm or 740 nm light (I = 20 µmol m −2 s −1 ) for 5 h and subsequently imaged using a transmission light <t>microscope</t> (scale bar = 200 µm). b Live cell imaging of cell–matrix interaction under 660 nm light and then switch to 740 nm light after 35 min with binary images of cell shapes to illustrate the cells spreading. For this, HeLa cells stably expressing OptoIntegrin were seeded on OptoMatrix and subsequently imaged. Micrographs were taken at indicated time points (scale bar = 10 µm). c Spatial control of cell attachment with OptoIntegrin-expressing HEK-293T cells. OptoIntegrin-expressing cells were cultivated on OptoMatrix, locally illuminated with 660 nm or 740 nm for 3 min and then left in darkness for 4 h. Afterwards, cells were fixed and imaged (scale bar = 200 µm)
    Upright Confocal Microscope Nikon Instruments Eclipse Ni E With A C2 Confocal Laser Scanner, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/confocal+microscope+nikon+eclipse+ni-e+c2/pmc06325061-167-24-27
    Average 90 stars, based on 1 article reviews
    upright confocal microscope nikon instruments eclipse ni-e with a c2 confocal laser scanner - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Validation of cell–matrix interaction with different cell lines stably expressing OptoIntegrin. a HEK-293T, HeLa and MCF7 cells stably expressing OptoIntegrin or moxGFP-PIF S were seeded on PhyB 1–651 -coated glass slides and incubated under 660 nm or 740 nm light (I = 20 µmol m −2 s −1 ) for 5 h and subsequently imaged using a transmission light microscope (scale bar = 200 µm). b Live cell imaging of cell–matrix interaction under 660 nm light and then switch to 740 nm light after 35 min with binary images of cell shapes to illustrate the cells spreading. For this, HeLa cells stably expressing OptoIntegrin were seeded on OptoMatrix and subsequently imaged. Micrographs were taken at indicated time points (scale bar = 10 µm). c Spatial control of cell attachment with OptoIntegrin-expressing HEK-293T cells. OptoIntegrin-expressing cells were cultivated on OptoMatrix, locally illuminated with 660 nm or 740 nm for 3 min and then left in darkness for 4 h. Afterwards, cells were fixed and imaged (scale bar = 200 µm)

    Journal: Communications Biology

    Article Title: Optogenetic control of integrin-matrix interaction

    doi: 10.1038/s42003-018-0264-7

    Figure Lengend Snippet: Validation of cell–matrix interaction with different cell lines stably expressing OptoIntegrin. a HEK-293T, HeLa and MCF7 cells stably expressing OptoIntegrin or moxGFP-PIF S were seeded on PhyB 1–651 -coated glass slides and incubated under 660 nm or 740 nm light (I = 20 µmol m −2 s −1 ) for 5 h and subsequently imaged using a transmission light microscope (scale bar = 200 µm). b Live cell imaging of cell–matrix interaction under 660 nm light and then switch to 740 nm light after 35 min with binary images of cell shapes to illustrate the cells spreading. For this, HeLa cells stably expressing OptoIntegrin were seeded on OptoMatrix and subsequently imaged. Micrographs were taken at indicated time points (scale bar = 10 µm). c Spatial control of cell attachment with OptoIntegrin-expressing HEK-293T cells. OptoIntegrin-expressing cells were cultivated on OptoMatrix, locally illuminated with 660 nm or 740 nm for 3 min and then left in darkness for 4 h. Afterwards, cells were fixed and imaged (scale bar = 200 µm)

    Article Snippet: Finally, the stained coverslips were mounted on microscope slides with Mowiol 4-88 (Carl Roth, cat. no.: 0713), and confocal images were acquired on an upright confocal microscope (Nikon Instruments Eclipse Ni-E with a C2 confocal laser scanner, 100 × oil objective NA = 1.45 or 60× oil objective NA = 1.40).

    Techniques: Biomarker Discovery, Stable Transfection, Expressing, Incubation, Transmission Assay, Light Microscopy, Live Cell Imaging, Control, Cell Attachment Assay